目的 建立赶黄草浸膏中同时测定5种成分的一测多评含量测定方法。方法 高效液相色谱法,采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×250 mm, 5 μm),流动相为乙腈-0.2%甲酸溶液梯度洗脱,流速1.0 mL·min-1,柱温30 ℃,检测波长280 nm。以乔松素-7-O-β-D-葡萄糖苷为内参物,建立赶黄草浸膏中该成分与没食子酸、短叶苏木酚酸、2, 6-二羟基苯乙酮-4-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-木糖苷及槲皮素-3'-O-α-L-鼠李糖苷的相对校正因子,进行含量测定,实现一测多评,同时采用外标法测定这些成分的含量,比较计算值与实测值的差异,验证所建方法的准确性和可行性。结果 21批赶黄草浸膏中,除短叶苏木酚酸外,其他5种成分采用校正因子计算的含量值与外标法实测值之间无显著差异,可以用一测多评法进行含量测定。结论 所建一测多评法可以用于赶黄草浸膏中特定的5种主要成分的含量测定。
Abstract
OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneous determination of five compounds in extract of Penthorum chinense Pursh. METHODS A HPLC method was established on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm, 5 μm). The mobile phase was acetonitrile-0.2% formic acid aqueous solution. Gradient elution was performed at a flow rate of 1.0 mL·min-1. The column temperature was 30 ℃ and the detection wavelength was set at 280 nm. Pinocembrin-7-O-β-D-glucoside was selected as the internal reference substance, and the relative correlation factors(RCF) of gallic acid, brevifolin carboxylic acid, 2, 6-dihydroxyacetophenone-4-O-β-D-glucoside, quercetin-3-O-β-D-xyloside and quercetin-3'-O-α-L-rhamnoside were determined by HPLC. The contents of the six components were determined by both QAMS method and external standard method and the RESULTS were compared. The accuracy and feasibility of the new method were validated by QAMS method and external standard method. RESULTS No significant differences were observed in the determination RESULTS of five constituents except brevifolin carboxylicacid in 21 batches of extract of P. chinense by QAMS method and external standard method. CONCLUSION The QAMS method is feasible and suitable to determine the contents of five constituents in the extract of P. chinense.
关键词
赶黄草 /
一测多评法 /
高效液相色谱法 /
相对校正因子
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Key words
Penthorum chinense Pursh. /
quantitative analysis of multi-components by single marker /
HPLC /
relative correction factor
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中图分类号：
R917
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参考文献
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